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目的建立一种定量检测食品中黄曲霉毒素的高效液相色谱方法。方法样品经提取、免疫亲和柱净化,以甲醇-水(45:55,v/v)为流动相,C18柱分离后,经柱后光化学衍生并通过荧光检测器定量。结果 4种黄曲霉毒素在25min内得到良好的基线分离,检出限0.25μg/kg,在1.0～10.0μg/KGg范围内,AFB1、AFB2、AFG1、AFG2加标回收率分别为94.2%～97.5%、95.3%～99.1%、84.5%～97.8%和88.4%～98.2%,相对标准偏差分别为3.6%～5.8%、3.5%～5.5%、4.6%～7.9%和4.3%～6.6%。结论该方法准确、简便、重复性好、灵敏度高,能满足食品中黄曲霉毒素定性定量检测要求。
Objective To establish a method for the quantitative determination of aflatoxins in food by high performance liquid chromatography. Methods The samples were extracted and purified by immunoaffinity column. The mobile phase was methanol - water (45:55, v / v) and separated on a C18 column. The column was photochemically derivatized and quantified by fluorescence detector. Results The four aflatoxins showed a good baseline separation within 25 min with a detection limit of 0.25 μg / kg. The recovery rates of AFB1, AFB2, AFG1 and AFG2 in the range of 1.0 ~ 10.0 μg / KGg were 94.2% ~ 97.5 %, 95.3% -99.1%, 84.5% -97.8% and 88.4% -98.2%, respectively. The relative standard deviations were 3.6% -5.8%, 3.5% -5.5%, 4.6% -7.9% and 4.3% -6.6% respectively. Conclusion The method is accurate, simple, reproducible and sensitive. It can meet the requirements of qualitative and quantitative detection of aflatoxins in food.