建立了反相高效液相色谱测定新生儿败血症患者血清中去甲万古霉素浓度的方法。血清样品经乙腈－异丙醇沉淀蛋白，上清液用三氯甲烷萃取后以水相进样。流动相为乙腈－磷酸盐缓冲液，紫外检测，最低检测限０．３ｍｇ／Ｌ，有良好的线性关系，回收率达９４．７％，日内、日间精密度分别为２．２３％和２．６２％。结果表明，方法简便、有效。 A method was established for the determination of norniccillin in serum of neonates with sepsis by reversed-phase high performance liquid chromatography. Serum samples were precipitated with acetonitrile-isopropanol, supernatants were extracted with chloroform and then injected as an aqueous phase. The mobile phase was acetonitrile-phosphate buffer, UV detection, the lowest detection limit of 0.3mg / L, a good linear relationship, the recovery rate of 94.7%, intraday and interday precision were 2.23% and 2 .62%. The results show that the method is simple and effective.