目的:改变发酵条件以提高发酵收率。方法:优化pH值、通风量、微量元素等发酵因子,找到最佳使用量。结果:发酵培养基中L-山梨糖起始浓度8%、碳酸钙浓度0.05%、复合VB以及分段调节pH值、通气量等可以有效地提高2-酮基-L-古龙酸的产量。结论:通过优化发酵条件可以优化发酵菌系的生理状态,促进大小菌之间的协调,使其达到最佳发酵状态。 Purpose: To change the fermentation conditions to improve the fermentation yield. Methods: Optimize the pH, ventilation, trace elements and other fermentation factors, find the best use. Results: The yield of 2-keto-L-gulonic acid could be effectively increased by adding 8% of L-sorbose and 0.05% of calcium carbonate in the fermentation medium. Conclusion: By optimizing the fermentation conditions, the physiological state of the fermentation bacteria can be optimized and the coordination between the bacteria can be optimized to reach the optimum fermentation state.