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目的:探讨人巨细胞病毒重组抗原在ELISA中的应用.方法:采用基因工程方法组建含有巨细胞病毒PPUL32及PPUL44蛋白共三个强抗原决定簇基因的重组抗原克隆,以此重组抗原建立ELISA方法,用于117份待测血清巨细胞病毒IGM抗体的检测,并将ELISA方法与免疫转印方法进行了比较.结果:经统计学分析,建立的ELISA方法与免疫转印检测方法的检测结果具有非常显著的一致性(χ2＝54.08,P＜0.01).与免疫转印方法比较,其敏感性和特异性分别为100％和81.3％;阳性预测率和阴性预测率分别为60.5％和100％,此ELISA方法具有良好的重复性.结论:此重组抗原具有良好的应用价值,可望替代HCMV感染ELISA诊断中的全病毒抗原.“,”Objective: Our aim was to evaluate the application of human cytomegalovirus(HCMV) recombinant antigen in enzymelinked immunosorbent assay(ELISA). Methods: A recombinant clone containing three strong antigenic epitope genes of HCMV PPUL 32 and PPUL 44 was constructed by genetic engineering method. The purified recombinant antigen was used in ELISA detect HCMV IgM antibodies for 117 cases of serum. The ELISA method was compared with immunoblotting test. ...