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以木薯(Manihot esculenta)栽培种‘华南5号’(SC5)为研究材料,利用双层黑塑料袋造成暗光环境,木薯在暗光环境分别生长0、1、3、9 d和暗光环境9 d后见光4 h,采用蛋白质组学方法,揭示与光合作用相关的蛋白质表达水平变化。结果表明:随着暗光环境处理天数的增加,叶绿素总含量整体呈下降趋势;以0 d暗光环境处理的木薯叶片蛋白质图谱为对照,通过其他暗光环境处理共得到45个平均差异表达量为对照±2.0倍以上的蛋白质,它们的生物学功能涉及光合作用、碳和能量代谢、结构、解毒、抗氧化等。而ATP合酶、核酮糖-1,5-二磷酸羧化酶/加氧酶(RuBisCO)活化酶、RuBisCO大亚基、磷酸核酮糖激酶前体、光系统I亚基及RuBisCO小亚基前体随着暗光环境处理天数延长而下调,显示这些蛋白质的表达水平与光合作用强弱密切相关。
Taking Manihot esculenta cultivar ’SC5’ as the research material, dark plastic bag was used to make dark environment, cassava was grown in dark environment for 0, 1, 3 and 9 d and dark environment After 9 days of light exposure to light for 4 h, proteomics was used to reveal changes in the protein expression levels associated with photosynthesis. The results showed that the total content of chlorophyll decreased with the increasing days of dark environment treatment. The protein profiles of cassava leaves treated with dark environment at 0 d were compared, and 45 different average light intensity As a control ± 2.0-fold higher than the protein, their biological functions related to photosynthesis, carbon and energy metabolism, structure, detoxification, antioxidant and so on. While ATP synthase, RuBisCO activator, RuBisCO macromolecule, phosphoribulokinase precursor, photoperiod I subunit and RuBisCO diminutive The reduction of base precursors with the prolonged days of dark light treatment showed that the expression level of these proteins was closely related to the intensity of photosynthesis.