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目的:建立UPLC-MS-MS同时测定大鼠血浆中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷,1-脱氧野尻霉素和决明子苷、红镰霉素龙胆二糖苷、橙黄决明素、甲基钝叶决明素、白藜芦醇的分析方法,研究大鼠服用滋肾清肝代平方后的药代动力学特征。方法:采用Waters Acquity UPLC BEH C18色谱柱,流动相0.1%甲酸水溶液-乙腈梯度洗脱,采用电喷雾电离源,扫描方式为多反应离子监测。结果:7种成分在大鼠血浆中线性关系良好,提取回收率94.83%~106.58%,日内、日间精密度和准确度良好。7种成分在模型组大鼠中的药时曲线下面积、末端半衰期、达峰时间、清除率、表观分布容积、药峰浓度分别为(326.65±26.66)μg·L-1·h-1,(3.64±1.69)h,0.33 h,(60.56±5.32)L·h-1·kg-1,(325.13±167.18)L·kg-1,(169.25±18.02)μg·L-1。结论:该方法特异、快速、准确、灵敏,可用于滋肾清肝代平方在大鼠体内的药动学研究。
OBJECTIVE: To establish a method for simultaneous determination of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, 1-deoxynojirimycin and cassia seed in rat plasma by UPLC-MS- Erythromycin gentianobiose glycosides, orange Huang Caiming, methyl blunt cassia seed, resveratrol analysis of rats to take Zishen Qinggan on behalf of the square after the pharmacokinetic characteristics. Methods: Waters Acquity UPLC BEH C18 column and mobile phase of 0.1% formic acid in water were used for gradient elution. The electrospray ionization source was used. The scanning mode was multi-reactive ion monitoring. Results: There was a good linear relationship between the seven components in rat plasma and the recoveries ranged from 94.83% to 106.58%. The precision and accuracy of intra-day and inter-day were good. The area under the curve, the terminal half-life, the peak time, the clearance rate, the apparent volume of distribution and the drug peak concentration of the seven components in the model group were (326.65 ± 26.66) μg · L -1 · h -1 , (3.64 ± 1.69) h, 0.33 h, (60.56 ± 5.32) L · h-1 · kg-1, (325.13 ± 167.18) L · kg-1 and (169.25 ± 18.02) μg · L- Conclusion: The method is specific, rapid, accurate and sensitive and can be used to study the pharmacokinetics of Zishen Qinggan Formula in rats.