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用乙型肝炎病毒单克隆表面抗体免疫新西兰兔制备抗独特型抗体，经小鼠γ球蛋白－ｓｅｐｈａｒｏｓｅ４Ｂ免疫亲和柱纯化后，用ＥＬＩＳＡ鉴定，结果表明特异性良好。以此抗独特型抗体免疫Ｂａｌｂ／ｃ小鼠，引起抗－ＨＢ＿ｓ应答，提示制备抗独特型疫苗的可能性。
Anti-idiotypic antibodies were prepared by immunizing New Zealand rabbits with monoclonal antibody against Hepatitis B virus. The antibodies were purified by mouse γ-globin-sepharose 4B affinity immuno-affinity column and identified by ELISA. The results showed good specificity. Balb / c mice were immunized with this anti-idiotypic antibody, eliciting an anti-HBs response, suggesting the possibility of preparing an anti-idiotypic vaccine.