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目的和方法：Ｈｅｙｍａｎｎ肾炎（ＨＮ）是一种用于研究人类膜性肾病的大鼠动物模型。用主动型ＨＮ模型大鼠的淋巴细胞与小鼠ＳＰ２／０骨髓瘤细胞融合，制备针对ＨＮ致病原的单克隆抗体ｃ３～６（ＭｃＡｂｃ３～６），并用它诱导大鼠被动型ＨＮ。结果：免疫酶组织化学及免疫电镜显示，ＭｃＡｂｃ３～６特异性识别肾小球上皮足突细胞膜抗原并与其结合形成免疫复合物。
PURPOSE AND METHOD: Heymann nephritis (HN) is a rat animal model used to study human membranous nephropathy. Monoclonal antibody c3 ~ 6 (McAbc3 ~ 6) against HN pathogen was prepared by fusion of mouse lymphocytes from mouse model of active HN with mouse SP2 / 0 myeloma cells and used to induce rat passive HN. Results: Immunohistochemistry and immunoelectron microscopy showed that McAb 3 ~ 6 specifically recognized the membrane antigen of glomerular epithelial cells and formed an immune complex.