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目的:将传统的外周血单个核细胞分离计数方法加以改进,并探讨2型糖尿病(DM)患者外周血单个核细胞数量与疾病的相关性。方法:密度梯度离心分离30例健康体检者单个核细胞,不同染色稀释液重悬细胞,充入改良牛鲍计数板连续计数5天,计算批内、批间变异;采用上述方法分离2型DM患者抗凝全血29例,计算单个核细胞数量;采用全自动生化分析仪检测对照组和2型DM组患者血脂。结果:两组的单个核细胞经3种前处理染液稀释重悬细胞后,计数结果间无统计学差异(P>0.05);2型DM组与健康对照组比较,外周血单个核细胞计数绝对值无显著性差异(P>0.05),血清TG、TC、LDL水平明显升高,差别具有统计学意义(P<0.05),而其他指标结果间比较无差异。在对照组中,单个核细胞计数结果与白细胞呈正相关(r=0.611,P<0.05),与血清TC、LDL水平呈负相关(r1=-0.550,r2=-0.605,P<0.05)。而T2DM组,单个核细胞计数结果仅与LDL水平呈负相关(r=-0.104,P<0.05)。结论:外周血单个核细胞数与2型糖尿病无明显相关性。
OBJECTIVE: To improve the method of separating and counting traditional peripheral blood mononuclear cells and investigate the relationship between the number of peripheral blood mononuclear cells and the disease in type 2 diabetes mellitus (DM). Methods: Mononuclear cells were isolated from 30 healthy subjects by density gradient centrifugation. Cells were resuspended with different staining diluents. The cells were filled with modified abalone counting plate for 5 consecutive days to calculate intra- and inter-batch variation. The type 2 DM Patients anticoagulant whole blood in 29 cases, counting the number of mononuclear cells; using automatic biochemical analyzer test control group and type 2 DM patients with blood lipids. Results: There was no significant difference in counting results between the two groups of mononuclear cells diluted by three pretreatment stains (P> 0.05). Compared with healthy control group, the number of peripheral blood mononuclear cells There was no significant difference between the absolute value (P> 0.05), serum TG, TC, LDL levels were significantly increased, the difference was statistically significant (P <0.05), while the other indicators were no difference between the results. In the control group, mononuclear cell count was positively correlated with leukocyte count (r = 0.611, P <0.05) and negatively correlated with TC and LDL levels (r1 = -0.550, r2 = -0.605, P <0.05). The T2DM group, mononuclear cell count results only negatively correlated with LDL levels (r = -0.104, P <0.05). Conclusion: There is no significant correlation between peripheral blood mononuclear cells and type 2 diabetes mellitus.