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细胞因子α干扰素 (IFNα)具有中枢镇痛作用。抗内源性阿片肽血清与IFNα能发生明显的交叉反应 ,提示IFNα与内源性阿片肽之间存在着共同的抗原决定基。采用基因定点突变技术 ,获得系列IFNα突变体 ,并分别测定其免疫学活性和镇痛能力。结果显示 ,IFNα突变体Y1 2 9S IFNα免疫学活性显著下降 ,但仍然保留了很强的镇痛能力 ,阿片受体拮抗剂纳洛酮能够阻断Y1 2 9S IFNα的镇痛作用。实验结果表明 ,IFNα分子存在着相互独立的免疫和镇痛两个功能位点 ,分别介导免疫调节作用和中枢镇痛作用。结果还提示 ,IFNα的免疫调节和中枢镇痛作用可能是由不同的受体途径介导的。
Cytokine interferon (IFNα) has a central analgesic effect. Anti-endogenous opioid peptide serum and IFNα significant cross-reaction occurred, suggesting that IFNα and endogenous opioid peptides exist between the common epitopes. A series of IFNα mutants were obtained by site-directed mutagenesis, and their immunological activity and analgesic ability were determined respectively. The results showed that the IFNα mutant Y1 2 9S IFNα significantly decreased the immunological activity, but still retained a strong analgesic ability. The opioid receptor antagonist naloxone blocked the analgesic effect of Y1 2 9S IFNα. Experimental results show that IFNα molecules exist independent of each other immune and analgesic two functional sites, respectively, mediated immune regulation and central analgesic effect. The results also suggest that IFNα immune regulation and central analgesic effects may be mediated by different receptor pathways.