论文部分内容阅读
土壤主要污染物中的重金属镉(Cd)已被列为优先关注污染物以及人类致癌物之一,会影响农作物的生长发育,并且可以通过食物链进入人体,影响人类健康。已有研究表明,在模式植物拟南芥和水稻中,Nramp蛋白家族在重金属镉吸收转运和调控过程中起到了关键作用,但在大豆中尚未有该基因参与重金属镉吸收和转运的报道。通过大豆数据库的分析,发现了一个Nramp家族基因,命名为Gm Nramp1(Glyma07g02680)。RT-PCR分析结果表明,在不同时间75μmol·L-1Cd Cl2处理的大豆根、子叶、胚轴和叶中Gm Nramp1基因均下调表达,而且在Cd处理的根系转录组测序分析中也得到了相似的结果。这些结果说明,Gm Nramp1基因可能在大豆中参与Cd吸收或转运过程。该研究为大豆抗重金属育种提供理论基础和基因资源。
Heavy metal cadmium (Cd), the main pollutant in soil, has been listed as one of the priority pollutants and human carcinogen, which will affect the growth and development of crops and enter the human body through the food chain, affecting human health. It has been reported that the Nramp family of proteins plays a key role in the transport and regulation of heavy metal cadmium in model plant Arabidopsis and rice, but no such gene has been reported to be involved in Cd uptake and transport in soya. An analysis of the soybean database revealed a Nramp family of genes, named Gm Nramp1 (Glyma07g02680). RT-PCR analysis showed that Gm Nramp1 gene was down-regulated in 75μmol·L-1Cd Cl2 treated soybean roots, cotyledons, hypocotyls and leaves at different time points, and was also similar in Cd-treated roots transcriptome sequencing analysis the result of. These results suggest that Gm Nramp1 may be involved in Cd uptake or transport in soybean. This study provides a theoretical basis and gene resources for anti-heavy metal breeding of soybean.