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目的：检测应用直接腹腔注射白细胞介素２（ＩＬ－２）重组腺病毒和（或）白细胞介素３（ＩＬ－３）重组腺病毒方法对化疗后小鼠造血功能恢复的影响。方法：正常小鼠腹腔注射大剂量环磷酰胺（２００ｍｇ／ｋｇ）２４小时后，直接腹腔注射ＩＬ－２重组腺病毒（Ａｄ－ＩＬ－２）和（或）ＩＬ－３重组腺病毒（Ａｄ－ＩＬ－３），用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）检测相应细胞内细胞因子ｍＲＮＡ，并持续观察小鼠外周血白细胞、股骨单个核细胞、骨髓ＣＦＵ－ＧＭ的恢复情况。结果：腹腔注射Ａｄ－ＩＬ－２和（或）Ａｄ－ＩＬ－３组小鼠腹腔细胞可测及相应细胞因子ｍＲＮＡ，血清中可测及相应细胞因子；腹腔注射Ａｄ－ＩＬ－３组小鼠外周血白细胞、股骨单个核细胞、骨髓ＣＦＵ－ＧＭ最低值均显著提高（Ｐ＜０．０５），但腹腔注射Ａｄ－ＩＬ－２组小鼠对化疗后小鼠造血功能无明显影响，联合应用Ａｄ－ＩＬ－２和Ａｄ－ＩＬ－３与单独应用Ａｄ－ＩＬ－３相比无显著性差异。结论：腹腔直接注射Ａｄ－ＩＬ－３能促进化疗后小鼠造血功能的恢复。
OBJECTIVE: To detect the effect of direct intraperitoneal injection of interleukin 2 (IL-2) recombinant adenovirus and / or interleukin 3 (IL-3) recombinant adenovirus on the recovery of hematopoietic function in mice after chemotherapy. Methods: The normal mice were injected intraperitoneally with high dose cyclophosphamide (200mg / kg) for 24 hours. The mice were injected intraperitoneally with Ad-IL-2 and / or IL- IL-3). The mRNA expression of cytokines was detected by reverse transcription-polymerase chain reaction (RT-PCR). The recovery of peripheral blood leukocytes, femur mononuclear cells and bone marrow CFU-GM was observed. Results: The intraperitoneal injection of Ad-IL-2 and / or Ad-IL-3 in mice peritoneal cells were measured cytokines and cytokines, measurable serum and corresponding cytokines; Ad-IL-3 intraperitoneal injection of mice The lowest value of peripheral blood leukocytes, femur mononuclear cells and bone marrow CFU-GM were significantly increased (P <0.05), but the mice injected with Ad-IL-2 ip had no significant effect on the hematopoietic function of mice after chemotherapy There was no significant difference between Ad-IL-2 and Ad-IL-3 compared with Ad-IL-3 alone. Conclusion: Direct intraperitoneal injection of Ad-IL-3 can promote the recovery of hematopoietic function in mice after chemotherapy.