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为了监测生防真菌绿僵菌菌株在田间释放后的回收,有必要建立菌株DNA分子标记,以此将应用的菌株与其他菌株或田间的土著分离株鉴别开来。作者采用16条随机引物扩增了51株绿僵菌菌株的基因组DNA,得到81个多态性位点。其中M189菌株多态性位点30个,分析得到1个特异性位点,并将该位点的DNA片段测序后转化成为特异性SCAR标记。检验确定了该标记的敏感性,可以从供试的51株菌株中准确鉴定出目的菌株M189。并用该标记检测了从田间回收的3个分离株,确定其中1个与应用菌株M189一致。
In order to monitor the recovery of the antifungal Metarhizium anisopliae strain after it has been released in the field, it is necessary to establish a strain of DNA molecular marker to identify the applied strain from indigenous isolates from other strains or fields. The authors used 16 random primers to amplify genomic DNA of 51 Metarhizium strains and obtained 81 polymorphic loci. Among them, there were 30 polymorphic loci in M189, and one specific locus was obtained. The DNA fragment of this locus was sequenced and transformed into a specific SCAR marker. The test confirmed the sensitivity of the marker, and the target strain M189 can be accurately identified from the 51 strains tested. The three isolates recovered from the field were detected using this marker, and one of them was confirmed to be consistent with the strain M189.