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为估价雌激素、雌激素受体(ER)对慢性鼻窦炎发生的作用,确定ER在鼻窦粘膜的分布部位,本文用鼠抗人单克隆抗ER抗体,对61例慢性鼻窦炎患者的前组筛窦粘膜,2例正常筛窦粘膜和10例正常鼻粘膜的石蜡标本行SLAB法免疫组化染色,同时作常规HE染色、甲苯胺蓝染色。结果发现ER主要在部分粘膜下腺体的腺泡和腺管上皮细胞表达,慢性鼻窦炎组筛窦粘膜ER阳性率为55.7％,2例正常鼻窦粘膜内未见ER阳性细胞,10例正常鼻腔粘膜ER阳性率为20％,ER阳性组嗜酸性细胞和肥大细胞浸润程度明显高于ER阴性组。结果提示雌激素、雌激素受体对慢性鼻窦炎发病有一定作用。
To evaluate the role of estrogen and estrogen receptor (ER) in the pathogenesis of chronic sinusitis and determine the distribution of ER in the sinus mucosa, we used mouse anti-human monoclonal anti-ER antibody to detect the expression of ER in 61 patients with chronic sinusitis Ethmoid sinus mucosa, 2 normal ethmoid mucosa and 10 normal nasal mucosa paraffin specimens were immunohistochemically stained with SLAB method. Meanwhile, HE staining and toluidine blue staining were performed. The results showed that ER was mainly expressed in acinar and glandular epithelium of some submucosal glands. ER positive rate of ethmoid sinus in chronic sinusitis group was 55.7%. There were no ER positive cells in 2 normal sinus mucosa and 10 normal nasal cavity Mucosal ER positive rate was 20%, ER-positive eosinophils and mast cells infiltration was significantly higher than the ER-negative group. The results suggest that estrogen and estrogen receptors play a role in the pathogenesis of chronic sinusitis.