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目的本研究是探讨丹参酮对离体肺保护的作用。方法以离体兔肺模型为肺保护实验对象,以肺动脉灌洗再次肺动脉灌注为实验方法,以新西兰兔为实验动物,随机分为两组。一组用LPD液行肺动脉灌洗和保存,另一组用丹参酮加LPD液组成实验组,用同样的方法灌洗和保存。保存18 h后,观察病理形态、含水量以及测量NO(一氧化氮)、SOD(超氧化物歧化酶)及MDA(丙二醛)的含量。结果肉眼见组织新鲜红润,部分组织充血、水肿及淤血;光镜下两组的肺泡、支气管及毛细血管结构完整,高倍视野下对照组较实验组肺泡上皮及毛细血管上皮细胞肿胀、肥大,少部分组织可见肺泡内有红细胞渗出、间隔增宽等。实验组的含水量低于对照组(P<0.05)。实验组肺组织中的NO及SOD含量高于对照组(P<0.05),MDA含量低于对照组(P<0.05)。结论丹参酮能清除氧自由基,有较强的抗氧化性,能较明显的减轻肺的再灌注损伤。在LPD液中加入丹参酮,能够对离体肺起到更好的保护效果。离体兔肺再灌注模型是一种较理想的实验动物模型。
Objective This study was to investigate the effect of tanshinone on isolated lung protection. Methods Rabbit lung model was used as the lung protection experiment subject. Pulmonary arterial lavage and pulmonary artery perfusion were used as experimental methods. New Zealand rabbits were used as experimental animals and randomly divided into two groups. One group was treated with LPD fluid for pulmonary lavage and the other group was treated with tanshinone plus LPD fluid to form an experimental group. The same method was used for lavage and preservation. After 18 h storage, the pathological morphology, water content, and NO (nitric oxide), SOD (superoxide dismutase), and MDA (malondialdehyde) content were measured. The results showed fresh red tissue, naked eyes, some tissue congestion, edema and congestion; under light microscope, the alveolar, bronchial and capillaries were intact in the two groups; in the high-power field, the alveolar epithelium and capillary epithelial cells in the control group were swollen, hypertrophied, and less than in the experimental group. Some tissues showed red blood cells in the alveoli, interval widening and so on. The water content of the experimental group was lower than that of the control group (P<0.05). The content of NO and SOD in the lung tissue of the experimental group was higher than that of the control group (P<0.05), and the content of MDA was lower in the experimental group than in the control group (P<0.05). Conclusion Tanshinone can scavenge oxygen free radicals, has strong antioxidant activity, and can significantly reduce lung reperfusion injury. The addition of tanshinone to the LPD solution provides better protection against isolated lungs. The isolated rabbit lung reperfusion model is an ideal experimental animal model.