Serex技术是基于体液免疫的重组cDNA文库的血清学分析方法,简便易行。其原理是利用分子克隆技术和将患者自体血清对抗原的自体分型技术融为一体,不仅可检测抗体反应,而且能在抗原与患者自体血清反应的基础上直接从分子水平确定具有免疫原性的抗原。此方法的流程主要是以新鲜组织或细胞样本构建cDNA文库并连接入噬菌体表达载体,以重组的噬菌体转染大肠杆菌, Serex technology is a serological assay based on the humoral immune recombinant cDNA library, which is simple and easy to perform. The principle is the use of molecular cloning technology and the patient’s autologous serum self-typing technology integration, not only detect the antibody response, but also in the antigen and the patient’s own serum on the basis of direct determination of the molecular level with the immunogenicity Of the antigen. The method is mainly based on the method of constructing a cDNA library from fresh tissue or cell sample and ligating into a phage expression vector, and then transfecting E. coli with the recombinant bacteriophage.