琼脂是制备母种培养基试管斜面的必要原料。但它价贵,且小城镇不易见到。为此,想用资源丰富、价廉易得的原料来代替琼脂制备母种培养基斜面。 (一) 材料与方法:试验揉用大米粉、面粉和木薯粉三种为原料,以马铃薯葡萄糖琼脂培养基为对照,共设四个处理。马铃薯葡萄糖琼脂培养基(PDA)的制作方法同一般,三种无琼脂培养基的制作方法如下:分 Agar is the necessary material to prepare the tube slant of the master culture medium. But it is expensive, and small towns are not easy to see. To this end, want to use resource-rich, cheap raw materials instead of agar to prepare mother-medium slope. (A) Materials and Methods: Three kinds of raw materials, rice flour, flour and tapioca flour, were tested and kneaded in potato dextrose agar medium for four treatments. The potato dextrose agar medium (PDA) was prepared in the same way as the three agarless mediums were as follows: