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在开展口腔医学基础课题的研究中,常需利用组织学方法来了解经实验动物的血管再生情况。观察动物颌面部的血管相,必须掌握颈总动脉灌注法,将各种能够着色的染料剂注入血管内,以使血管腔着色而便于观察。经颈总动脉灌注墨汁来观察组织的血管化和再血管化,是一种应用较广的基础实验方法。该种方法操作简便,设备简单,成本低,效果好,实用价值高,目前这一方法的应用文献中尚未见有专题介绍。自1982年以来,作者应用该法分别观察了狗、兔在行自体冷冻骨移植术、异体胚胎骨移植术、下齿槽神经移植术、下颌前牙根尖下截骨术后各种组织的血管和微血管改变,获得了较为满意的效果,现以兔为实验动物,将我们的方法和体会介绍如
In the study of the basic subjects of stomatology, often require the use of histological methods to understand the experimental animals, the situation of vascular regeneration. Observe the animal maxillofacial vascular phase, we must master the common carotid artery perfusion method, will be able to dye a variety of dye into the blood vessels in order to make the vascular cavity coloring and easy to observe. Through the common carotid artery perfusion ink to observe the organization of vascularization and revascularization, is a widely used basic experimental method. The method is simple in operation, simple in equipment, low in cost, good in effect and high in practical value. At present, there are no special presentations in the applied literature of this method. Since 1982, the authors observed the dogs and rabbits in various tissues such as autologous frozen bone transplantation, allogeneic embryo implantation, inferior alveolar nerve grafting, mandibular anterior apical osteotomy And microvascular changes, obtained a more satisfactory results, the rabbit is now used as experimental animals, will introduce our methods and experiences such as