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目的通过建立肾阳虚大鼠垂体-甲状腺轴和垂体-肾上腺轴相关生物分子的支持向量机模型,探讨肾阳虚过程中甲状腺与肾上腺功能的动态关系。方法肌肉注射氢化可的松复制肾阳虚大鼠模型,分别测定早期、中期和晚期肾阳虚大鼠垂体-甲状腺轴生物分子TSH,T3,T4以及垂体-肾上腺轴生物分子ACTH,CORT的含量,建立TSH,T3,T4与ACTH、CORT之间的支持向量回归机模型,利用模型分析垂体-甲状腺轴与垂体-肾上腺轴生物分子之间的相互调控规律。结果当垂体-甲状腺轴3个生物分子指标值下降时,垂体-肾上腺轴2个生物分子指标值早期明显下降,中期稍有上升,晚期大幅下降;当垂体-肾上腺轴两个生物分子指标值下降时,垂体-甲状腺轴3个生物分子的指标值早期有不同程度的上升,中期下降,晚期较大程度下降。并且所建支持向量回归机模型留一法检验的相对误差都在10%以内。结论垂体-甲状腺轴生物分子与垂体-肾上腺轴生物分子之间的相互调控失衡是肾阳虚的发病机制之一。同时说明支持向量回归机模型能比较准确反映肾阳虚大鼠甲状腺与肾上腺腺功能之间复杂的非线性关系。
Objective To establish a support vector machine model for pituitary-thyroid axis and pituitary-adrenal axis related biomolecules in kidney-yang deficiency rats, and to explore the dynamic relationship between thyroid gland and adrenal gland function in the course of kidney-yang deficiency. Methods Hydrocortisone was intramuscularly injected into rat model of kidney-yang deficiency, and the contents of TSH, T3, T4 and ACTH, CORT of pituitary-thyroid axis biomolecules in pituitary-adrenal axis were measured in early, middle and late-stage kidney-yang deficiency rats. A support vector regression model was established between TSH, T3, T4 and ACTH and CORT, and models were used to analyze the mutual regulation between pituitary-thyroid axis and pituitary-adrenal axis biomolecules. Results When the values of three biomolecules in the pituitary-thyroid axis decreased, the values of two biomolecules in the pituitary-adrenal axis decreased significantly in the early stage, slightly increased in the middle period, and decreased significantly in the late stage; when the values of two biomolecules in the pituitary-adrenal axis decreased At the time, the index values of the three biomolecules in the pituitary-thyroid axis increased in different degrees in the early stage, decreased in the middle stage, and decreased in the late stage. In addition, the relative error of the one-off test of the support vector regression model built is within 10%. Conclusion The imbalance between the pituitary-thyroid axis biomolecules and the pituitary-adrenal axis biomolecules is one of the pathogenesis of kidney-yang deficiency. At the same time, the support vector regression model can accurately reflect the complex nonlinear relationship between thyroid gland and adrenal gland function in kidney-yang deficiency rats.