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以魔芋茎尖、幼芽为外植本,接种于1/2MS+1.0mg/LBA+0.01mg/LNAA的培养基中,茎尖、幼芽逐渐生长直接形成幼芽或幼苗;或从茎尖、幼芽由来的膨大的块茎组织表面诱导出幼芽。膨大的块茎组织分割后接种于MS+0.01mg/L+0.01mg/LNAA的培养基中进行增殖培养,同时从增殖的块茎组织表面不断地诱导出幼芽。幼芽切块转入不含激素的MS培养基中,形成幼苗。幼苗切块转入MS+0.1mg/LNAA的生根培养茎中,幼苗生根,形成完整的植株。试管苗移栽6个月后获得干块茎。
The shoots and shoots were gradually grown to shoots or shoots with the tips of shoots and shoots of Amorphophallus as explants inoculated with 1 / 2MS + 1.0mg / LBA + 0.01mg / L NAA. Buds originate from the enlarged tuber tissue surface inducing shoots. The enlarged tuber tissue was dissected and inoculated into a medium supplemented with MS + 0.01 mg / L + 0.01 mg / L NAA to proliferate and culture, while buds were continuously induced from the surface of proliferating tuber tissue. Shoots were transferred into hormone-free MS medium to form seedlings. Seedlings were cut into MS + 0.1mg / LNAA rooting culture stems, seedlings rooted to form a complete plant. Test tube seedlings after transplanting 6 months to obtain dry tubers.