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采用营养液培养法,研究了外源Ca(NO3)2对Na Cl胁迫下黄瓜幼苗生长和生理指标的影响。结果表明:60mmol·L-1Na Cl胁迫显著抑制了幼苗形态生长和生物量积累,叶片光合色素和抗坏血酸含量显著降低,质膜透性、丙二醛、脯氨酸和可溶性蛋白含量显著增加;不同浓度Ca(NO3)2处理,均使Na Cl胁迫下叶片光合色素和抗坏血酸含量显著增加,质膜透性和MDA含量显著降低,脯氨酸和可溶性蛋白含量进一步增加,幼苗生长各指标不同程度提高,其中4 mmol·L-1Ca(NO3)2处理的各项指标均优于2 mmol·L-1和6 mmol·L-1Ca(NO3)2处理。说明外源Ca(NO3)2可通过促进叶片光合色素合成,增强细胞抗氧化能力和渗透调节能力,减轻胁迫造成的膜脂过氧化伤害,缓解Na Cl胁迫对黄瓜幼苗生长的抑制,且以4mmol·L-1Ca(NO3)2处理的缓解效果最好。
The nutrient solution culture method was used to study the effects of exogenous Ca (NO3) 2 on the growth and physiological indices of cucumber seedlings under NaCl stress. The results showed that: 60 mmol·L-1 NaCl stress significantly inhibited morphological growth and biomass accumulation of seedlings, significantly decreased leaf photosynthetic pigment and ascorbic acid content, plasma membrane permeability, malondialdehyde, proline and soluble protein content; The contents of photosynthetic pigments and ascorbic acid, the content of plasma membrane and MDA were significantly decreased, the content of proline and soluble protein was further increased and the indexes of seedling growth were increased to some extent , Among which the indexes of 4 mmol·L-1Ca (NO3) 2 were better than those of 2 mmol·L-1 and 6 mmol·L-1Ca (NO3) 2. The exogenous Ca (NO3) 2 could alleviate the inhibition of NaCI stress on the growth of cucumber seedlings by promoting photosynthetic pigment synthesis, enhancing cell antioxidant and osmoregulation ability, reducing the damage of membrane lipid peroxidation caused by stress, · L-1Ca (NO3) 2 treatment is the best.