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应用抗c-myc癌基因产物(P62~(c-myc))单克隆抗体(Mycl-9E10)做免疫组织化学染色,观察了50例人大肠癌标本中P62~(c-myc)的表达水平及其分布。在50例人大肠癌病例中,有32例为中度或高度分化,被列入高分化组,其余18例被分入低分化组。标本在手术室取材后立即在中性福尔马林溶液中固定,以保证组织的完整性。50例标本中有39例为P62~(c-myc)阳性染色,阳性率78%。阳性染色主要分布在肿瘤细胞的胞质中,偶见细胞核染色,而且染色的强弱、范围、分布及其阳性百分率与肿瘤细胞的分化程度无关。实验结果提示:P62~(c-myc)在肿瘤细胞中分布广泛,在大多数人大肠癌细胞中有P62~(c-myc)的过度表达,而且这种过度表达的意义尚需进一步探讨。
The expression of P62 c-myc in 50 human colorectal carcinomas was detected by immunohistochemistry with anti-c-myc gene product (P62 c-myc) monoclonal antibody (Mycl-9E10) And its distribution. In 50 cases of human colorectal cancer cases, 32 cases were moderately or highly differentiated, were included in well-differentiated group, and the remaining 18 cases were divided into poorly differentiated group. Specimens were taken in the operating room and immediately fixed in neutral formalin solution to ensure the integrity of the tissue. Among 50 specimens, 39 cases were positive for P62 c-myc, the positive rate was 78%. Positive staining mainly in the cytoplasm of tumor cells, and occasionally staining the nucleus, and the staining of the strength, scope, distribution and the positive percentage has nothing to do with the degree of differentiation of tumor cells. The experimental results suggest that P62 c-myc is widely distributed in tumor cells and overexpression of P62 c (c-myc) in most human colorectal cancer cells, and the significance of this overexpression remains to be further explored.