报道了Ｎ_１′－甲基靛玉红肟的合成、晶体结构及性质．体外美蓝试验结果表明，它的抗白血病活性明显优于靛玉红，其水溶性和脂溶性都较靛玉红有所增强．用Ｘ射线衍射法测得其晶体结构，组成为Ｃ_（１７）Ｈ_（１３）Ｎ_３Ｏ_２，Ｎ_ｒ＝２９１．２１，空间群Ｐ２_１／ｃ，晶体学数据：ａ＝１．５２２６（４），ｂ＝０．６５２４（３），ｃ＝１．６５６２（５）ｎｍ；β＝１０３．６９（４）°，Ｚ＝４．最终偏离因子为０．０４３．分子近似平面型． The synthesis, crystal structure and properties of N 1’-methyl indirubin oxime were reported. In vitro methylene blue test results show that its anti-leukemia activity was significantly better than indirubin, its water-soluble and fat-soluble than indirubin increased. The crystal structure was determined by X-ray diffractometry. The crystal structure was C_ (17) H_ (13) N_3O_2, N_r = 291.21 and space group P2_1 / c. The crystallographic data were: a = 1.5226 0.6524 (3), c = 1.6562 (5) nm; β = 103.69 (4) °, Z = 4. The final deviation factor is 0.043. Molecular approximate planar.