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目的通过免疫组织化学技术检测N-cadherin、CD44、CD133和Nanog在卵巢上皮性肿瘤组织中的表达情况,探讨N-cadherin与肿瘤干细胞标志物间的相互关系及临床意义。方法采用免疫组织化学En Vision法分别检测N-cadherin、CD44、CD133和Nanog在卵巢上皮性癌(EOC)及卵巢良性肿瘤中的表达。结果 N-cadherin在EOC中的阳性表达率为66.7%(20/30),高于在卵巢良性肿瘤中的阳性表达率16.7%(5/30),差异有统计学意义(P<0.05);N-cadherin阳性表达与TNM分期、淋巴结转移、组织学分级有关(P<0.05),与年龄无关(P>0.05);N-cadherin与CD44表达呈显著相关(r=0.523,P<0.05)。结论 N-cadherin在EOC的发生发展中发挥重要作用。N-cadherin可能与CD44参与了肿瘤细胞干性的生成,引起了肿瘤的转移与复发。
Objective To detect the expression of N-cadherin, CD44, CD133 and Nanog in ovarian epithelial tumor by immunohistochemistry and to explore the relationship between the expression of N-cadherin and tumor stem cell markers and their clinical significance. Methods The expressions of N-cadherin, CD44, CD133 and Nanog in epithelial ovarian cancer (EOC) and benign ovarian tumor were detected by Envision immunohistochemistry. Results The positive expression rate of N-cadherin in EOC was 66.7% (20/30), which was higher than that in benign ovarian tumors (16.7%, 5/30). The difference was statistically significant (P <0.05). The positive expression of N-cadherin was correlated with TNM stage, lymph node metastasis and histological grade (P <0.05), but not with age (P> 0.05). There was a significant correlation between the expression of N-cadherin and CD44 (r = 0.523, P <0.05). Conclusion N-cadherin plays an important role in the development of EOC. N-cadherin may be involved in the formation of CD44 dry tumor cells, causing tumor metastasis and recurrence.