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目的探索表面增强激光解析离子化飞行时间质谱技术(SELDI)在传染性非典型肺炎又称[严重急性呼吸综合征(SARS)]早期诊断中的应用价值。方法用SELDI检测SARS患者组和对照组血清,建立诊断模型,然后进行单盲模型验证。结果通过检测37例SARS患者和74名健康对照者血清,发现早期SARS患者血清中有4种特异性蛋白质荷比峰(M/Z)-3939.08、4137.71、8136.64和11514.20,其中4137.71下调,另3种上调,并以此建立SARS诊断模型,对37例SARS患者和73名对照者血清进行单盲检测,敏感性为97.3%(36/37),特异性为91.8%(67/73)。结论用SELDI可从早期SARS患者中筛选出4个特异蛋白质荷比峰,以此建立的诊断模型可配合临床对SARS患者进行早期诊治。
Objective To explore the value of surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI) in the early diagnosis of SARS, also known as Severe Acute Respiratory Syndrome (SARS). Methods SELDI was used to detect the serum of patients with SARS and the control group, and the diagnostic model was established. Then the single blind model was used to verify the results. Results Serum samples from 37 patients with SARS and 74 healthy individuals were detected. There were 4 specific protein peak (M / Z) -3939.08, 4137.71, 8136.64 and 11514.20 in the sera of early SARS patients, of which 4137.71 was down-regulated while the other 3 In order to establish a SARS diagnostic model, the sera of 37 patients with SARS and 73 controls were detected by single blind method. The sensitivity and specificity were 97.3% (36/37) and 91.8% (67/73) respectively. Conclusion SELDI can screen four specific protein peak from early SARS patients. The diagnostic model established by this method can be used in clinical diagnosis and treatment of SARS patients early.