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在我们过去的研究中,曾对葡萄嫁接后砧穗间的相互影响作过一些工作,也曾就此发表过几篇有关文章。本文仅就接穗对砧木根系的过氧化物酶活性、呼吸速率及可溶性蛋白的影响,进行了测试分析。1 材料与方法1.1 材料取自净月果园的4年生巨峰、红香水、康太、罗也尔实生葡萄品种的嫁接苗和自根苗的根系,进行有关生理指标的测试分析。嫁接苗砧木为贝达（Beta）,葡萄园管理水平一般。取样时间为10月20日。1.2 测定方法1.2.1用联苯胺比色法测根系过氧化物酶1.2.2 用红外仪法测根系呼吸速率。1.2.3 用考马斯亮兰G250法测根的可溶性蛋白。2结果与分析2.1根系过氧化物酶活性 过氧化物酶（POD）是活性较高的一种酶,它与光合作用、呼吸作用以及生长素的氧化等都有密切关系。在植物生长发育过程中,
In our previous studies, we have done some work on the interaction between grapes and grapes after grafting and also published a few articles on this. This paper only on the scion rootstocks peroxidase activity, respiration rate and soluble protein, were tested and analyzed. 1 Materials and methods 1.1 Materials taken from the net orchard of 4 months Kyoho, Hong Perfume, Health too, Roeger grape varieties of grafted roots and roots of the root system, the physiological indicators of the test analysis. Grafted seedlings for the Beta (Beta), vineyard management level in general. Sampling time for the October 20. 1.2 Determination 1.2.1 benzidine spectrophotometric method for the determination of root peroxidase 1.2.2 measured by infrared spectroscopy root respiration rate. 1.2.3 with Coomassie brilliant blue G250 method to measure the root of soluble protein. 2 Results and Analysis 2.1 Root Peroxidase Activity Peroxidase (POD) is a highly active enzyme, which is closely related to photosynthesis, respiration and the oxidation of auxin. In the process of plant growth and development,