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背景:胰岛素样生长因子1是一种多肽类激素,已证明其对前体细胞增殖有促进作用。目的:探讨静脉注射胰岛素样生长因子1对大鼠脑缺血后神经干细胞增殖、迁移和分化的影响。方法:成年雄性SD大鼠80只,随机分为对照组和实验组,40只/组。两组大鼠均采用改良线栓法制备局灶性脑缺血模型,实验组大鼠通过尾静脉注射胰岛素样生长因子1,按100μg/kg计算,连续注射6d;对照组给予等剂量的生理盐水。分别于干预后7,14,21,28d断头去脑,各组分别在处死前1d腹腔注射BrdU。采用免疫组织化学及其双重染色法检测BrdU阳性细胞、PSA-NCAM阳性细胞、BrdU+PSA-NCAM双阳性细胞、BrdU+MAP2双阳性细胞的表达。结果与结论:BrdU阳性细胞、PSA-NCAM阳性细胞数均在缺血后7d最多;BrdU+PSA-NCAM双标阳性细胞在缺血后双侧室管膜下区和海马齿状回区均可以检测到,于7d计数最多,之后逐渐减少;BrdU+MAP2双阳性细胞却从14d开始逐渐增多,随BrdU+PSA-NCAM双阳性表达的逐渐降低,BrdU+MAP2双阳性表达逐渐增高,呈现此消彼涨的变化。提示静脉途经给予胰岛素样生长因子1能诱导大鼠缺血性脑损伤后神经干细胞的增殖、分化和迁移。
BACKGROUND: Insulin-like growth factor 1 is a polypeptide hormone that has been shown to promote progenitor cell proliferation. Objective: To investigate the effects of insulin-like growth factor-1 on the proliferation, migration and differentiation of neural stem cells after cerebral ischemia in rats. Methods: Eighty adult male Sprague-Dawley rats were randomly divided into control group and experimental group, 40 rats / group. Rats in both groups were given focal cerebral ischemia model by modified suture method. Rats in experimental group were injected with insulin-like growth factor 1 through the tail vein at a dose of 100 μg / kg for 6 days. The rats in control group were given equal doses of physiology brine. The rats were sacrificed on the 7th, 14th, 21st and 28th day after the intervention, respectively, and the rats in each group were intraperitoneally injected with BrdU one day before sacrifice. The expression of BrdU positive cells, PSA-NCAM positive cells, BrdU + PSA-NCAM double positive cells and BrdU + MAP2 double positive cells were detected by immunohistochemistry and double staining. RESULTS AND CONCLUSION: The positive cells of BrdU positive cells and PSA-NCAM cells were all the highest at 7 days after ischemia. The double positive cells of BrdU + PSA-NCAM in bilateral subependymal zone and dentate gyrus of hippocampus The positive rate of BrdU + MAP2 double positive cells gradually increased from the 14th day to the 2nd day after BrdU + PSA-NCAM double positive expression, and the double positive expression of BrdU + MAP2 increased gradually He rose up the change. Prompt intravenous administration of insulin-like growth factor 1 can induce neural stem cell proliferation, differentiation and migration after ischemic brain injury in rats.