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本实验对切除颌下腺３５天、５２天和７０天的小鼠做了睾丸生精细胞ＤＮＡ含量的流式细胞分析．结果表明：①各实验组的１Ｃ细胞群（精子细胞和精子）和４Ｃ细胞群（初级精母细胞）比例明显低于相应的对照组（Ｐ＜０．０１），２Ｃ细胞群（精原细胞和次级精母细胞等）比例明显高于对照组（Ｐ＜０．０１）．②实验组各峰的均道值低于对照组（Ｐ＜０．０１），即ＤＮＡ含量低于对照组．③细胞周期分析显示实验组的Ｇ０／Ｇ１期和Ｓ期细胞比例增加（Ｐ＜０．０１），Ｇ２／Ｍ期细胞比例明显减少（Ｐ＜０．０１），同时ＰＩ值明显降低（Ｐ＜０．０１）。上述结果提示体内表皮生长因子（Ｅｐｉｄｅｒｍｅｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）缺乏可导致小鼠睾丸初级精母细胞和精子细胞减少，并可影响细胞的ＤＮＡ合成和延缓生精细胞的增殖．
In this study, flow cytometry analysis of DNA content of testicular germ cells was performed on mice resected for 35 days, 52 days and 70 days. The results showed that: ① The proportion of 1C cell population (spermatozoa and spermatozoa) and 4C cell population (primary spermatocyte) in each experimental group was significantly lower than that of the corresponding control group (P <0.01) And secondary spermatocytes, etc.) was significantly higher than the control group (P <0.01). ② The mean value of each peak in the experimental group was lower than that of the control group (P <0.01), that is, the DNA content was lower than that of the control group. ③ The cell cycle analysis showed that the proportion of cells in G0 / G1 phase and S phase in experimental group increased (P <0.01), the percentage of cells in G2 / M phase decreased significantly (P <0.01), while the PI value decreased significantly (P < 0.01). The above results suggest that lack of epidermal growth factor (EGF) in vivo may lead to the reduction of primary spermatocytes and spermatids in testis and may affect DNA synthesis and delay the proliferation of spermatogenic cells.