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目的 为探讨丹参对庆大霉素耳毒性的减毒作用。方法 将 2 4只沙鼠分为 4组 ,每组 6只 ,①生理盐水对照组。②丹参组 (SA)。③庆大霉素加丹参组 (GM +SA)。④庆大霉素组 (GM)。所有药物均经肌肉注射 ,GM组和GM +SA组每天注射庆大霉素 15 0mg/kg ,共 10d ;GM +SA组和SA组同时注射丹参 2 g/kg ,共 10d ;生理盐水组每天注射生理盐水 2ml/kg ,共 10d。在注射前和注射后 4周和 8周进行CAP检测 ,第 8周听力测定后处死动物行扫描电镜检查。结果 4周和 8周GM组高频CAP阈明显上升 ,而GM +SA组听阈明显低 ,统计学处理有显著差异 (P <0 .0 1,t检验 ) ;GM组毛细胞损伤率底圈和第二圈分别为 35 %和 30 % ,GM +SA组分别为 9%和 7% ,差异非常显著 (P <0 .0 1,χ2 检验 )。结论 丹参能明显降低庆大霉素对沙鼠的耳毒性作用 ,且不降低血液中庆大霉素的浓度。电生理检测结果与形态学变化一致。
Objective To explore the attenuated effect of Salvia miltiorrhiza on gentamicin ototoxicity. Methods 24 gerbils were divided into 4 groups, 6 in each group. ① Saline control group. ② Salvia group (SA). ③ gentamicin plus Salvia group (GM + SA). ④ gentamicin group (GM). All the drugs were injected intramuscularly, GM and GM + SA groups were injected with gentamicin 150 mg / kg every day for 10 days. Salvia miltiorrhiza 2 g / kg was injected into GM + SA group and SA group simultaneously for 10 days. Injection of saline 2ml / kg, a total of 10d. CAP test was performed before injection and at 4 weeks and 8 weeks after injection. At the 8th week after the hearing test, the animals were sacrificed by scanning electron microscopy. Results At 4 weeks and 8 weeks, the high-frequency CAP threshold was significantly increased in GM group, while the threshold in GM + SA group was significantly lower than that in GM group (P <0.01, t test) And 35% and 30% respectively in the second lap and 9% and 7% in the GM + SA group respectively (P <0.01, χ2 test). Conclusion Danshen can significantly reduce gentamicin ototoxicity in gerbils, and does not reduce the concentration of gentamicin in the blood. Electrophysiological test results and morphological changes.