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本文报告了大鼠骨髓中核酸的荧光定量测定方法,对于实验条件进行了探讨,应用激发光波长为520nm,荧光波长为590nm,EB与DNA,骨髓匀浆络合物的荧光光谱完全一致,标准DNA或RNA含量与荧光强度是直线相关,DNA标准回收率为99.5%,方法误差系数为5.7%,灵敏度为0.025γ/ml。对10只正常成年大鼠骨髓测定结果,DNA均值15.2γ/mg组织(或8.7Pg/细胞),RNA均值3.0γ/mg组织。此法也可用于测定白细胞DNA含量,10只大鼠白细胞DNA均值为10.4Pg/细胞。
In this paper, we reported the fluorescence quantitative determination of nucleic acids in bone marrow of rats. The experimental conditions were discussed. The excitation light wavelength was 520nm and the fluorescence wavelength was 590nm. The fluorescence spectra of EB, DNA and bone marrow homogenate complexes were identical. The standard The DNA or RNA content is linearly correlated with the fluorescence intensity. The standard DNA recovery was 99.5% with a method error of 5.7% and a sensitivity of 0.025γ / ml. The results of bone marrow determination of 10 normal adult rats showed that the mean of DNA was 15.2γ / mg (or 8.7Pg / cell) and the mean RNA was 3.0γ / mg. This method can also be used to determine leukocyte DNA content, 10 rat white blood cells mean 10.4Pg / cell.