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目的：评价葡萄膜与结膜色素性肿瘤细胞ＤＮＡ含量及增殖活性在其良恶性肿瘤鉴别或恶性程度判断上的价值。方法：应用流式细胞术（ｆｌｏｗｃｙｔｏｍｅｔｒｙ，ＦＣＭ）对葡萄膜恶性黑色素瘤（ｕｖｅａｌｍａｌｉｇｎａｎｔｍｅｌａｎｏｍａｓ，ＵＭＭ）、结膜色素痣（ｃｏｎｊｕｎｃｔｉｖａｌｎｅｖｉ，ＣＮ）及恶性黑色素瘤（ｃｏｎｊｕｎｃ－ｔｉｖａｌｍａｌｉｇｎａｎｔｍｅｌａｎｏｍａｓ，ＣＭＭ）的ＤＮＡ含量、增殖指数（ｐｒｏｌｉｆｅｒａｔｉｖｅｉｎｄｅｘ，ＰＩ）及Ｓ期细胞百分率（ｓ－ｐｈａｓｅｆｒａｃｔｉｏｎ，ＳＰＦ）进行检测。结果：７例ＣＮ均为二倍体，７例ＣＭＭ及３９例ＵＭＭ的异倍体率分别为５７．１４％、７４．３６％。ＣＭＭ的ＳＰＦ和ＰＩ显著高于ＣＮ的ＳＰＦ（Ｐ＜０．０５）和ＰＩ（Ｐ＜０．０１）；ＵＭＭ的ＤＮＡ指数（ＤＮＡｉｎｄｅｘ，ＤＩ）、异倍体率与病理类型均表现一定的相关性（Ｐ＜０．０１）。１９例ＵＭＭ的ＤＩ与核仁组成区相关嗜银蛋白（ａｒｇｙｒｏｐｈｉｌｉｃｎｕｃｌｅｏｌａｒｏｒｇａｎｉｚｅｒｒｅｇｉｏｎｓａｓｓｏｃｉａｔｅｄｐｒｏ－ｔｅｉｎｓ，ＡｇＮＯＲｓ）银染面积呈显著正相关（Ｐ＜０．０１）。结论：研究结果提示异倍
Objective: To evaluate the DNA content and proliferative activity of uveal and conjunctival pigmented tumor cells in the differential diagnosis of benign and malignant tumors or the value of malignancy. Methods: The DNA content, proliferative index (VEGF) of uvealmalignant melanomas (UMM), conjunctival nevi (CN) and conjunctival melanoma (CMM) were measured by flow cytometry (FCM) proliferative index (PI) and s-phase fraction (SPF). Results: All 7 CN patients were diploid. The aneuploidy rates of 7 CMM and 39 UMM were 57.14% and 74.36% respectively. The SPF and PI of CMM were significantly higher than that of CN (P <0.05) and PI (P <0.01). The DNA index (DI) and aneuploidy rates of UMM were correlated with pathological types (P <0.01). There was a significant positive correlation between DI of 19 cases of UMM and the area of silver staining of argyrophilic nucleolar organizers associated with proteins (AgNORs) (P <0.01). Conclusion: The results suggest that aneuploidy