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为明确广东地区传播木尔坦棉花曲叶病毒(Cotton leaf curl Multan virus,CLCu Mu V)的烟粉虱隐种,采用分子鉴定、烟粉虱传毒试验与分子检测的方法,对传播CLCu Mu V的烟粉虱隐种进行了鉴定。结果表明:在广东棉花、红麻和黄秋葵曲叶病发生的田间,2014年采集的30头烟粉虱mt COI与Asia II 7隐种序列同源性为98.3%~99.6%,2015年采集的10头烟粉虱中,8头烟粉虱mt COI与MEAM1隐种序列同源性为99.2%~99.5%,2头烟粉虱mt COI与Asia II 7隐种序列同源性为99.8%~99.9%,说明烟粉虱种群包括入侵隐种MEAM1和土著隐种Asia II 7。在利用烟粉虱人工传毒试验中,MEAM1、Asia II 7和Asia II 1这3个隐种均可在棉花曲叶病株上饲毒获得CLCu Mu V及β卫星分子;除MEAM1隐种外,Asia II 7、Asia II 1隐种可传播CLCu Mu V,侵染红麻及黄秋葵植株引起曲叶病,前者传毒效率分别为50%和100%;而后者为33%和100%。以上3个烟粉虱隐种传毒接种棉花未见成功。
In order to clarify the prevalence of B. tabaci hidden in Cotton Cultivars (CLCu Mu V) in Guangdong Province, molecular identification, transmission assay and molecular detection of B. tabaci were used to detect CLCu Mu V whitefly was identified. The results showed that the homology of the mtDNA of 30 B. tabaci populations with that of Asia II 7 was 98.3% ~ 99.6% in the fields of cotton, kenaf and okra leafhopper occurred in Guangdong in 2014, Among 10 whitefly Bemisia tabaci, the homology of mt COI and MEAM1 in eight B. tabaci was 99.2% -99.5%. The homology of mt COI of two B. tabaci and that of Asia II 7 was 99.8% 99.9%, indicating that the population of Bemisia tabaci includes the invasive invaders MEAM1 and indigenous hidden Asia II 7. In the artificial transmission experiment of Bemisia tabaci (Gennadius), three recessive species, MEAM1, Asia II 7 and Asia II 1, could be infected with CLCu Mu V and beta satellite molecules on cotton leaf curl strains. Except MEAM1, , Asia II 7, Asia II 1 can spread CLCu Mu V, the infection of kenaf and Okra caused leaf curl disease, the former transmission efficiency were 50% and 100%; the latter was 33% and 100%. More than 3 Bemisia tabaci vaccination cotton inoculated without success.