[Objective] To establish a simple,rapid and accurate analytical method for detecting quercetin in Gynostemma pentaphyllum(Thunb.) Makino by high perfomance liquid chromatograph(HPLC).[Method]The conditions of chromatography were as follows:Microsorb-MV100-5C18 column(4.6 mm × 250 mm,5 m) was adopted with a mobile phase of methanol-mixtrue [acetonitrile:1% acetic acid:0.4% phosphoric acid,V/V/V](37:63,V/V);the flow rate was 0.6 ml/min,and the detective wavelength was 372 nm.[Result]The linear range was 0.64-25.60 g/ml(r = 0.999 9),the average recovery was over 96%(n = 9).[Conclusion] The established method is simple,rapid and accurate. [Objective] To establish a simple, rapid and accurate analytical method for detecting quercetin in Gynostemma pentaphyllum (Thunb.) Makino by high perfomance liquid chromatography (HPLC). [Method] The conditions of the chromatography were as follows: Microsorb-MV100-5C18 column (4.6 mm × 250 mm, 5 m) was adopted with a mobile phase of methanol-mixtrue [acetonitrile: 1% acetic acid: 0.4% phosphoric acid, V / V / V] flow rate was 0.6 ml / min, and the detective wavelength was 372 nm. [Result] The linear range was 0.64-25.60 g / ml (r = 0.999 9), the average recovery was over 96% (n = 9) Conclusion] The established method is simple, rapid and accurate.