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目的了解哈尔滨市病毒性腹泻的病原组成及其流行情况,为疾病防控提供依据。方法 2010年6-10月在哈尔滨市儿童医院和哈尔滨市第六医院采集疑似病毒性腹泻患者粪便样本44份,使用轮状病毒、腺病毒、星状病毒、诺如病毒和札如病毒特异性引物,采用PCR、套式PCR或一步RT-PCR方法进行病毒核酸检测,进而分析病原构成。结果共检测样本44份,其中A组轮状病毒G9型2份,腺病毒1份,星状病毒2份,诺如病毒GⅠ型1份,诺如病毒GⅡ型1份,札如病毒1份,病原检测阳性率为18.18%,没有混合感染。结论 PCR方法用于腹泻病毒检测特异、快速、敏感、易于分型,在44份病毒性腹泻患者样本中共检测到5种腹泻病毒。提示哈尔滨市病毒性腹泻病原复杂。
Objective To understand the etiological composition and prevalence of viral diarrhea in Harbin and provide evidence for disease prevention and control. Methods Forty-four samples of patients with suspected viral diarrhea were collected from Harbin Children’s Hospital and Harbin No.6 Hospital from June to October in 2010, using rotavirus, adenovirus, astrovirus, norovirus and saetavirus Primer, using PCR, nested PCR or one-step RT-PCR virus nucleic acid detection, and then analyze the pathogenic constitution. Results A total of 44 samples were tested, including 2 rotavirus group G9, 1 adenovirus, 2 astrovirus, 1 Norovirus GⅠ, 1 Norovirus GⅡ, 1 Sapporo virus , The positive rate of pathogen detection was 18.18%, there was no mixed infection. Conclusion The PCR method is specific, rapid, sensitive and easy to typed for detection of diarrhea virus. Five types of diarrhea virus were detected in 44 samples of patients with viral diarrhea. Tip Harbin virus diarrhea pathogenic complex.