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应用图象分析术及核仁组成区银染技术,对50例肾细胞病细胞核进行了多项参数测定和核仁组成区嗜银蛋白(Ag-NORs)检测.结果表明,肾癌的各项参数和Ag-NORs数量与病理分级、临床分期、细胞类型及预后密切相关;特别是DNA含量、核面积、核直径及Ag-NORs数量随病理分级或临床分期的上升而明显提高,在存活超过了3年与存活不足3年的病例之间的差异有高度统计意义(P<0.01),说明图象分析参数及Ag-NORs数量可为肾癌预后的判定提供辅助资料。进一步对肾癌DNA含量与Ag-NORs数量变化行直线相关分析,发现二者呈正相关。
Using image analysis and silver staining technique of nucleolar organizer region, 50 parameters of nucleus of renal cell disease and Ag-NORs of nucleolar organizer region were detected. The results showed that the parameters of renal cell carcinoma and the number of Ag-NORs were closely related to the pathological grade, clinical stage, cell type and prognosis. Especially, the content of DNA, nuclear area, nuclear diameter and the number of Ag-NORs were correlated with pathological grade or clinical stage (P <0.01), indicating that the number of image analysis parameters and the number of Ag-NORs may be the prognosis of renal cell carcinoma Determine the provision of supporting information. Further analysis of DNA content of renal cell carcinoma and Ag-NORs linear correlation analysis showed a positive correlation between the two.