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人体端粒DNA重复单元GGGTTA在K+溶液中可以形成G-四链体结构,并已成功利用结构变化过程中的荧光改变进行K+的检测。在此基础上研究了在寡聚核苷酸的GGG部分插入腺嘌呤碱基A对G-四链体结构及在K+检测中的影响。借助圆二色光谱、质谱和荧光光谱的方法可以分别获得序列变化引起的G1四链体排列的平行或反平行结构,可以结合的K+数目,以及在K+传感时所适用的K+浓度范围。在GGG中插入A可以使K+更容易进入G-四链体分子空穴,但当在两个GGG中同时插入A时,G-四链体结构难以形成。基于K+线性响应范围的变化(由0.02~1 mmol/L变为0.2~16 mmol/L),可以改进基于DNA的K+传感器,使直接测定人体血清中K+的含量成为可能。
Human telomere DNA repeat unit GGGTTA can form G-quadruplex structure in K + solution and the detection of K + has been successfully used for fluorescence change during structural change. Based on this, we investigated the effect of adenine-A insertion on the G-quadruplex structure in the GGG part of the oligonucleotide and in K + detection. Circular dichroism, mass spectrometry and fluorescence spectroscopy can be used to obtain parallel or antiparallel structures of G1 quadruplexes due to sequence changes, the number of K + that can be bound, and the range of K + concentrations that can be used for K + sensing, respectively. The insertion of A into GGG makes it easier for K + to enter the G-quadruplex molecular cavity, but the G-quadruplex structure is difficult to form when A is inserted into both GGGs simultaneously. Based on the change in K + linear response range from 0.02 to 1 mmol / L to 0.2 to 16 mmol / L, a DNA-based K + sensor can be modified to make it possible to directly determine K + in human serum.