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目的探讨ＨＰＶ１６Ｅ６基因ＤＮＡ诱发体液和细胞免疫反应的能力。方法利用基因工程技术构建了ＨＰＶ１６Ｅ６真核表达质粒ｐｃＤＮＡ３／Ｅ６，脂质体法转染Ｃｏｓ７细胞，肌肉注射免疫ＢＡＬＢ／ｃ小鼠，免疫组化技术检测抗体产生及抗原表达。结果被转染的Ｃｏｓ７细胞表达ＨＰＶ１６Ｅ６蛋白免疫小鼠产生抗ＨＰＶ１６Ｅ６抗体。结论这一结果为ＨＰＶ１６相关宫颈癌治疗性ＤＮＡ疫苗的研制提供了资料。
Objective To investigate the ability of HPV16E6 DNA to induce humoral and cellular immune responses. Methods The eukaryotic expression plasmid pcDNA3 / E6 of HPV16E6 was constructed by genetic engineering and transfected into Cos7 cells by lipofection. The BALB / c mice were injected intramuscularly, and the antibody production and antigen expression were detected by immunohistochemistry. Results The transfected Cos7 cells expressed HPV16E6 protein to immunize mice to produce anti-HPV16E6 antibody. Conclusion This result provides information for the development of therapeutic DNA vaccine for HPV16 related cervical cancer.