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目的 观察小鼠对 IL- 12基因表达质粒与 HBV S基因疫苗联合免疫的免疫应答 .方法 用已构建的 HBV S基因疫苗 (p CR3.1- S)和表达 IL- 12 p35和 p40蛋白的真核表达载体 p WRG316 9分别给 BAL B/c小鼠多点肌肉注射 ,2 wk后追加免疫 1次 ,用 EL ISA法及 MTT法检测小鼠血清抗 - HBs及脾细胞对 HBs Ag的特异性增殖反应 .结果 免疫接种 2 wk后小鼠血清抗 - HBs滴度及脾细胞对 HBs Ag的刺激指数均明显高于对照组 ,p WRG316 9+ p CR3.1- S组明显高于单纯p CR3.1- S注射组 .结论 IL- 12表达质粒可以增强 p CR3.1-S基因疫苗诱导的体液和细胞免疫应答强度 ;尤以细胞免疫增高明显
Objective To observe the immune response of mice combined immunization with IL-12 gene expression plasmid and HBV S gene vaccine.Methods The constructed recombinant vaccine of HBV S gene (p CR3.1-S) and the recombinant protein of IL-12 p35 and p40 The BALB / c mice were injected intramuscularly with p WRG3169, and immunized twice a week after immunization. The serum anti-HBs and splenocytes were detected by ELISA and MTT method. Proliferative response.Results After 2 weeks of immunization, the serum anti-HBs titer and the stimulation index of spleen cells to HBsAg were significantly higher than that of the control group, and p WRG316 9+ p CR3.1-S group was significantly higher than that of p CR3 .1-S injection group.Conclusion IL-12 expression plasmid can enhance the strength of humoral and cellular immune response induced by p CR3.1-S gene vaccine, especially the cellular immunity increased obviously