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This paper evaluated the antifungal activity and mechanism of the main active component of the root extract of Tagetes(Tagetes fungicide),and an emulsion was developed to improve water solubility of Tagetes fungicide,thus improving its application.The results of in vitro antifungal effect assay and scanning electron microscopy(SEM)showed that the Tagetes fungicide had a wide spectrum of fungicidal ability and the anti-fungal mechanism of fungicide might be associated with a change in hyphae morphology.The total protein expression of Fusarium oxysporum f.sp.Niveum changed after being cultured with fungi-cide,showing the decreased expression of high molecular protein while increasing expression of low molecular protein.When the hydrophilic-lipophilic balance value was 10,the emulsion was stable.The intracellular organelles of plant leaves infected by F oxysporum f.sp.Niveum showed some changes after cultured with the fungicide emulsion.