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[目的]探讨谷胱甘肽S转移酶基因多态性对1-溴丙烷(1-BP)接触者神经传导速度的影响。[方法]选择116名1-BP接触工人为接触组,133名无1-BP接触史的工人为对照组,用神经电生理检测技术测定工人的运动神经传导速度(MCV)、感觉神经传导速度(SCV)和末端潜伏期(DL),用等位基因特异性扩增(ASA)方法检测两组GSTT1和GSTM1基因型的分布。[结果]GSTT1基因多态性和1-BP接触对工人的SCV和DL的影响有交互作用,SNK两两比较结果显示,与对照组GSTT1基因携带者相比,接触组中GSTT1基因缺失型工人的SCV明显减慢[分别为(45.3±5.0)m/s和(40.1±5.3)m/s]、DL明显延长[分别为(6.3±1.3)ms和(8.3±2.0)ms],差别有统计学意义(P<0.05);去除交互项后的两因素方差分析显示,GSTT1基因多态性对工人的MCV有独立的影响(P<0.05)。未发现GSTM1基因多态性对接触者外周神经传导速度有影响。[结论]GSTT1基因多态性与接触者对1-BP外周神经毒性的易感性有关。
[Objective] To investigate the effect of glutathione S-transferase gene polymorphism on the nerve conduction velocity in 1-bromopropane (1-BP) contact. [Methods] A total of 116 workers with 1-BP exposure as contact group and 133 workers without history of 1-BP exposure as control group, the motor nerve conduction velocity (MCV), sensory nerve conduction velocity (SCV) and terminal latency (DL). The distribution of GSTT1 and GSTM1 genotypes in both groups was detected by allele-specific amplification (ASA). [Results] There was interaction between GSTT1 gene polymorphism and 1-BP exposure on SCV and DL in workers. SNK comparison showed that GSTT1 gene deletion workers in contact group were significantly lower than those in control group (45.3 ± 5.0) m / s and (40.1 ± 5.3) m / s, respectively, significantly prolonged DL [6.3 ± 1.3 ms vs 8.3 ± 2.0 ms, respectively] Statistical analysis (P <0.05). Two-way analysis of variance (ANOVA) revealed that GSTT1 gene polymorphism had an independent effect on MCV in workers (P <0.05). GSTM1 gene polymorphism was not found on the contact peripheral nerve conduction velocity. [Conclusion] GSTT1 gene polymorphism is related to susceptibility of 1-BP peripheral neurotoxicity in contact subjects.