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目的通过对乳腺癌患者外周血淋巴细胞染色体不稳定性的研究,来探讨乳腺癌患者外周血淋巴细胞中染色体畸变率(Chromosomal aberration,CAR)、染色体脆性部位(frahgle site)、姐妹染色单体交换(sister chromatid exchanges,SCE)、微核发生率(micronuclei,MN)及核仁组织形成区(Nucleolus organizer region,NOR)的相关性。方法对84例乳腺癌患者和110例正常人进行外周血染色体培养,常规收获细胞、制片,观察正常人和乳腺癌患者的染色体畸变率、染色体脆性部位、姐妹染色单体交换率、微核发生率、核仁组织形成区,并比较结果。结果 184例乳腺癌患者染色体结构畸变出现较多的的是1、3、5、6、10、11、17。2CAR、SCE频率、MN、AgNOR分别为11.07%、12.76±1.73、2.42±0.68‰、42.74%。3乳腺癌CAR、fra、SCE频率、MN、AgNOR各指标间呈正相关。结论乳腺癌患者外周血淋巴细胞染色体存在着数目及结构畸变,染色体畸变是导致乳腺癌发生的重要原因。肿瘤患者淋巴细胞染色体不稳定性的发生是其必然的表现,通过染色体不稳定性检测有助于发现高危患者。
Objective To investigate the chromosomal aberration (CAR), the frahgle site, the sister chromatid exchange in peripheral blood lymphocytes of patients with breast cancer by studying the chromosomal instability of peripheral blood lymphocytes in patients with breast cancer. (sister chromatid exchanges, SCE), micronuclei (MN) and nucleolus organizer region (NOR). Methods Eighty-four breast cancer patients and 110 healthy controls were cultured in peripheral blood and routinely harvested for cell growth. The chromosomal aberration rate, chromosomal fragility, sister chromatid exchange rate, micronucleus Incidence, formation of nucleolar organizers, and comparison of results. Results The chromosome aberrations in 184 cases of breast cancer patients were more common than that of the other cases (1,3,5,6,10,11,17.2CAR). The frequencies of SCE, MN and AgNOR were 11.07%, 12.76 ± 1.73 and 2.42 ± 0.68 ‰, respectively , 42.74%. 3 breast cancer CAR, fra, SCE frequency, MN, AgNOR indicators were positively correlated. Conclusion The chromosome number of peripheral blood lymphocytes in patients with breast cancer is the number and structural distortion, and chromosome aberration is the important cause of breast cancer. Tumor patients with lymphocyte chromosome instability is its inevitable manifestation of detection of chromosomal instability can help identify high-risk patients.