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目的 :探索广西地区壮、汉两族人群ApoB基因多态性的分布特点及其与冠心病、脑梗塞发病之间的关系。方法 :应用聚合酶链反应 (PCR)对广西壮族 17名冠心病患者 (CHD)、14名动脉粥样硬化性脑梗塞患者 (ACI)和 62名健康人的ApoB基因的XbaI、EcoRI位点的限制性片段长度多态性 (RFLP)进行了研究。结果 :无论是广西壮族人群冠心病组、脑梗塞组与正常对照组在XbaI位点以X- X- 为主要基因型 ,少见X+ X+ 、X+ X- 基因型 ;在EcoRI位点以E+ E+ 为主要基因型 ,少见E+ E- 、E- E- 基因型。X+ 等位基因在壮族三组人群中的频率分别为 :0 117、0 0 714、0 0 4 92 ,两两之间没有显著性差异 (P >0 0 5 ) ;E-在广西壮族三组人群中的频率为 :0 0 3 85、0 10 7、0 0 4 84 ,两两之间的差异未达统计学意义 (P >0 0 5 )。结论 :广西壮族人群冠心病、脑梗塞的发病与XbaI、EcoRI位点等位基因的分布没有明显的关联
Objective: To explore the distribution of ApoB gene polymorphisms in Zhuang and Han populations in Guangxi and its relationship with coronary heart disease and cerebral infarction. Methods: PCR was used to detect the XbaI and EcoRI sites of ApoB gene in 17 patients with coronary heart disease (CHD), 14 patients with atherosclerotic cerebral infarction (ACI) and 62 healthy individuals from Guangxi Zhuang Autonomous Region. Restriction Fragment Length Polymorphism (RFLP) was studied. Results: X-X- was the major genotype of XbaI locus in both coronary heart disease group and cerebral infarction group in Guangxi Zhuang population, but X + X + and X + X genotypes were rare in XbaI locus. E + E + The main genotype, rare E + E-, E-genotype. The frequencies of X + allele in Zhuang three groups were 0 117,0 0 714,0 0 4 92 respectively, with no significant difference between the two groups (P> 0.05) The frequency of the crowd: 0 0 3 85,0 10 7,0 0 4 84, the difference between every two was not statistically significant (P> 0 0 5). Conclusion: The incidence of coronary heart disease and cerebral infarction in Guangxi Zhuang population is not significantly associated with the distribution of alleles at XbaI and EcoRI loci