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目的探讨艾滋病病毒1型B′亚型(HI V-1 B′)慢性感染人群连续两年血浆中和能力的变化,及其与疾病进展的关系。方法通过对两年前后127例HI V-1 B′亚型感染者进行CD4和病毒载量(VL)的检测,采用基于TZM-b1细胞的中和实验方法,检测感染者血浆分别针对HI V-1临床分离株1597和实验室适应株SF33两种病毒的中和能力。通过分析中和50%病毒进入细胞的血浆浓度(EC50)与CD4计数和VL的相关性,阐明血浆中和水平与疾病进展的关系。结果2005年和2007年CD4分别为(454±131)个/μl和(440±182)个/μl(t=1.02,P=0.309),VL(log10)分别为(4.03±0.89)拷贝/ml和(4.21±0.89)拷贝/ml(t=2.72,P=0.008);血浆对1 597株有中和活性的分别有91例(71.65)和75例(66.93%),其EC50分别为(65.15±0.51)和(55.59±0.59)(t=1.16,P=0.248);对SF33株有中和活性的分别有108例(85.04%)和101例(85.04%),其EC50为(205.65±0.52)和(139±0.47)(t=3.57,P<0.001)。聚类分析显示,EC50与VL的关联性较强,但EC50的变化与VL的变化的相关性没有统计学意义(SF33∶r=0.07,P=0.41;1597∶r=0.12,P=0.18)。结论从2005年到2007年,慢性HI V-1B′亚型感染者的VL显著升高,尽管对病毒的中和水平降低,但血浆中和水平的降低与VL的升高没有直接的相关性。
Objective To investigate the changes of plasma neutralizing capacity in chronic infection group of HIV-1 B ’subtype (HI V-1 B’) for two consecutive years and its relationship with disease progression. Methods The CD4 and viral load (VL) were detected in 127 cases of HI V-1 B ’subtype two years before and after treatment. The TZM-b1 cell-based neutralization assay was used to detect the plasma levels of HI V -1 clinical isolate 1597 and the laboratory adapted strain SF33. The relationship between plasma neutralization and disease progression was elucidated by analyzing the correlation of plasma concentrations (EC50) of 50% neutralizing virus entry into cells with CD4 count and VL. Results CD4 (454 ± 131) / μl and (440 ± 182) / μl (t = 1.02, P = 0.309) and VL (log10) were 4.03 ± 0.89 copies / ml (4.21 ± 0.89) copies / ml (t = 2.72, P = 0.008). There were 91 (71.65) and 75 (66.93% ± 0.51) and (55.59 ± 0.59), respectively (t = 1.16, P = 0.248). There were 108 (85.04%) and 101 (85.04% ) And (139 ± 0.47) (t = 3.57, P <0.001). Cluster analysis showed that there was a strong correlation between EC50 and VL, but the correlation between EC50 and VL was not statistically significant (SF33:r = 0.07, P = 0.41; 1597:r = 0.12, P = 0.18) . Conclusions From 2005 to 2007, the VL of chronic HI V-1B ’subtype was significantly increased. Although the neutralization level of the virus was decreased, there was no direct correlation between the decrease of plasma neutral level and the increase of VL .