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本研究从香椿叶中提取分离多酚,研究多酚对糖尿病关键酶(猪胰腺α-淀粉酶、鼠小肠蔗糖酶和麦芽糖酶)活性的影响。采用活性追踪法,运用多种分离纯化技术和色谱学分析,逐级筛选活性强的部位以及单体化合物。研究发现香椿叶多酚粗提物具有显著的酶抑制作用,猪胰腺α-淀粉酶、鼠小肠蔗糖酶和麦芽糖酶的半数抑制率(IC50)值分别为0.45 mg/mL、1.29 mg/mL和1.41 mg/mL。乙酸乙酯萃取后的70%乙醇洗脱部位为主要活性部位,其对猪胰腺α-淀粉酶、鼠小肠蔗糖酶和麦芽糖酶抑制作用的IC50值分别为0.091mg/mL、0.97 mg/mL和1.00 mg/mL。从活性部位分离出2个单体化合物,分别鉴定为:1,2,3,4,6-O-五没食子酰葡萄糖(PGG)和没食子酸乙酯(EG),其中PGG具有显著的α-淀粉酶抑制活性,IC50值为0.069 mg/mL;EG对蔗糖酶和麦芽糖酶具有一定的抑制活性,IC50值分别0.82 mg/mL和0.81 mg/mL。研究表明多酚化合物是香椿叶的有效降血糖功能成分之一,PGG和EG是主要的活性成分。
In this study, polyphenol extracted from Toona sinensis leaves, polyphenols on the key enzymes of diabetes (porcine pancreatic α-amylase, small intestine sucrase and maltase) activity. Active tracking method, using a variety of separation and purification techniques and chromatographic analysis, step by step screening of active sites and monomer compounds. The results showed that the crude extract of Toona sinensis leaf polyphenol had significant enzyme inhibition. The IC50 values of porcine pancreatic α-amylase, mouse small intestine invertase and maltase were 0.45 mg / mL, 1.29 mg / mL and 1.41 mg / mL. The elution fraction of 70% ethanol extracted with ethyl acetate was the main active site. The IC50 value of inhibitory effect on porcine pancreatic α-amylase, small intestine invertase and maltase were 0.091mg / mL, 0.97 mg / mL and 1.00 mg / mL. Two monomer compounds were isolated from the active site and identified as 1,2,3,4,6-O-galloylglucose (PGG) and gallic acid ethyl ester (EG), respectively, in which PGG had significant α- The amylase inhibitory activity (IC50) was 0.069 mg / mL. EG had some inhibitory activity on sucrase and maltase with IC50 values of 0.82 mg / mL and 0.81 mg / mL, respectively. Studies have shown that polyphenolic compounds is one of the effective hypoglycemic components of Toona sinensis leaves, PGG and EG are the main active ingredients.