论文部分内容阅读
目的 :探讨反复熔铸对钴铬烤瓷合金生物相容性的影响。方法 :将钴铬烤瓷合金分别经过1~3次熔铸,使用DMEM细胞培养液进行浸提并采用电感耦合等离子体原子发射光谱仪(ICP-AES),检测浸提液中金属离子的种类和水平,使用合金浸提液培养小鼠肺结缔组织成纤维细胞(L-929),采用流式细胞仪检测L-929细胞周期的改变,Annexin-V-FITC/PI法检测L-929细胞凋亡与死亡情况。采用SPSS 13.0软件包对数据进行统计学分析。结果 :经过反复熔铸后,第3代合金与第1、第2代相比,除镓(Ga)的析出量增高外(P<0.05),其他金属离子的析出量及金属离子析出的总量均无显著变化(P>0.05);第1~3代合金对L-929细胞周期的影响无显著差异,早期凋亡率、晚期凋亡与坏死率差异均无显著性(P>0.05)。结论:经1~3次熔铸的钴铬烤瓷合金生物相容性未发生明显改变。
Objective: To investigate the effect of repeated casting on the biocompatibility of cobalt-chromium ceramic alloy. Methods: Cobalt-chromium porcelain alloys were cast one to three times respectively and then were extracted with DMEM cell culture medium. The species and level of metal ions in the extract were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) (L-929) was cultured in the leaching solution of the mouse lung. The cell cycle of L-929 cells was detected by flow cytometry. The apoptosis of L-929 cells was detected by Annexin-V-FITC / PI assay And the death situation. Data were statistically analyzed using SPSS 13.0 software package. Results: Compared with the first generation and the second generation, the third generation alloy produced more castings than the first generation and the second generation except for the increased amount of gallium (P <0.05), the amount of other metal ions precipitated and the amount of metal ions precipitated (P> 0.05). There was no significant difference in the cell cycle of L-929 cells between the first and the third generation of alloys. There was no significant difference in the rates of early apoptosis, late apoptosis and necrosis (P> 0.05). Conclusion: The biocompatibility of cobalt-chromium porcelain alloy cast by 1 ~ 3 times did not change obviously.