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[目的]研究肝炎平对肝纤维化大鼠肝细胞神经递质受体的作用,探讨其抗肝纤维化的可能机制。[方法]将38只Wistar大鼠分为3组:正常对照(N)组8只、肝炎平(G)组15只及模型(M)组15只。采用光镜及电镜观察肝组织病理学改变;免疫组织化学SP法检测各组肝组织M1型胆碱能受体(M1-AChR)和β2肾上腺素能受体(β2-AR)的表达水平。[结果]G组病理改变较M组明显改善,G组M1-AChR的表达较M组降低而β2-AR的表达较M组显著升高(P<0.05)。[结论]肝炎平能通过抑制肝组织M1-AChR的表达同时增加β2-AR的表达,从而调节神经递质在肝纤维化中的作用。
[Objective] To study the effect of Hepatitis on hepatocyte neurotransmitter receptor in hepatic fibrosis rats and explore the possible mechanism of hepatic fibrosis. [Methods] 38 Wistar rats were divided into 3 groups: 8 in the normal control (N) group, 15 in the Hepatitis (G) group, and 15 in the model (M) group. Light microscopy and electron microscopy were used to observe the histopathological changes of the liver. Immunohistochemical SP method was used to detect the expression of M1-type cholinergic receptor (M1-AChR) and β2-adrenergic receptor (β2-AR) in liver tissue of each group. [Results] The pathological changes in group G were significantly improved compared with those in group M. The expression of M1-AChR in group G was lower than that in group M and the expression of β2-AR was significantly higher than that in group M (P<0.05). [Conclusion] Heping can regulate the expression of M1-AChR in liver tissue and increase the expression of β2-AR, so as to regulate the role of neurotransmitter in hepatic fibrosis.