Changes in the weight and water content of the growth substrate at different cultivation times significantly affected Hypsizygus marmoreus growth yields.Highest growth yields(132.6~146.3 g per culture) were achieved when the mycelium culture and maturation period was between 80 and 90 days,and the substrate water content was 73%~74%.Physiological maturation of H.marmoreus mycelium was correlated with the substrate water content. Changes in the weight and water content of the growth substrate at different cultivation times significantly affected Hypsizygus marmoreus growth yields.Highest growth yields (132.6 ~ 146.3 g per culture) were achieved when the mycelium culture and maturation period was between 80 and 90 days, and the substrate water content was 73% ~ 74%. Physiological maturation of H. marmoreus mycelium was correlated with the substrate water content.