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DNA免疫不仅可诱生特异性体液免疫反应,还可诱生特异性CTL,因此可能在抗肿瘤免疫、抗病毒感染和治疗自身免疫疾病中起重要作用。增强DNA免疫诱生CTL反应可通过增强DNA的摄入、降低外源DNA在内质体中的降解以及加强内源性抗原多肽向内质问的转运从而增强MHC Ⅰ类限制的抗原呈递等方面来实现。相应的新策略有:利用EB病毒载体系统或偶联受体特异配基增强DNA摄入,加入内质体破坏多肽(病毒包膜糖蛋白或衣壳蛋白来源的双歧性多肽),降低外源DNA在细胞内的降解,应用内质网转运信号序列增强抗原多肽向内质网的转运。
DNA immunization can not only induce specific humoral immune response, but also induce specific CTL, which may play an important role in antitumor immunity, antiviral infection and the treatment of autoimmune diseases. Enhanced DNA immunization induced CTL response can be enhanced by increasing DNA intake, reducing the degradation of exogenous DNA in the endoplasmic reticulum, and strengthening the transport of endogenous antigen peptides to enhance the MHC class I restricted antigen presentation achieve. Corresponding new strategies are: use of Epstein-Barr virus vectors or coupled receptor-specific ligands to enhance DNA uptake, add endosomes to disrupt polypeptides (viral envelope glycoproteins or capsid protein-derived bidentate peptides), reduce external Degradation of the source DNA in the cell, using the endoplasmic reticulum transport signal sequence to enhance the transport of antigen polypeptide to the endoplasmic reticulum.