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AIM:To detect the thymidine phosphorylase(dThdPase)expression in human colorectal cancer tissues and cells.METHODS:Forty specimens resected from patients withcolorectal cancer were immunohistochemically stainedby 654-1,anti-dThdPase monoclonal antibody,PG-M1,anti-macrophage marker CD68 monoclonal antibody.Morphometrical analysis and positive cell counting wereperformed.In 27 of 40 specimens,dThdPase activity wasalso assayed by HPLC.Otherwise,the dThdPase level wasmeasured by ELISA in 6 colorectal cancer cell lines,LS174T,Clone A,Colo320,CX-1,Lovo,and MIP101,as well as in 2macrophage-like cell lines,THP-1 and U937.RESULTS:dThdPase activity was significantly increased incancer tissues compared with adjacent normal tissue(P<0.01).In immunohistochemical analysis,it was confirmed that mostcells expressed dThdPase were the stromal cells surroundingcancer nests or along the invasive margin of cancer.Basedon their morphometrical characteristics,we found that mostof them were tumor-associated macrophages(TAMs).Thenumber of dThdPase-positive stromal cells was significantlycorrelated with the number of CD68-positive macrophages(r=0.76,P<0.0001).By ELIS4,18.2 unit/mg and 19.3 unit/mgof dThdPase protein were detected in THP-1 and U937,butonly little was detected in 6 colorectal cancer cell lines.CONCLUSION:The present data suggest that dThdPaseexpression is seldom detected in colorectal carcinoma cells.TAM is the most important source of dThdPase in colorectalcancer tissues.
AIM: To detect the thymidine phosphorylase (dThdPase) expression in human colorectal cancer tissues and cells. METHODS: Forty specimens resected from patients with colorectal cancer were immunohistochemically stainedby 654-1, anti-dThdPase monoclonal antibody, PG- M1, anti-macrophage marker CD68 monoclonal antibody. Morphometrical analysis and positive cell counting were performed. 27 of 40 specimens, dThdPase activity was assayed by HPLC. Still, the dThdPase level wasmeasured by ELISA in 6 colorectal cancer cell lines, LS174T, Clone A, Colo320, CX- 1, Lovo, and MIP101, as well as in 2 macrophage-like cell lines, THP-1 and U937. RESULTS: dThdPase activity was significantly increased incancer tissues compared with adjacent normal tissue (P <0.01) .In immunohistochemical analysis, it was confirmed that most cells expressed dThdPase were the stromal cells surrounding cancer nests or along the invasive margin of cancer. Based on their morphometrical characteristics, we found that most of them were tumor-associated m (T = 0.76, P <0.0001) .By ELIS4, 18.2 unit / mg and 19.3 unit / mg of dThdPase protein were detected in THP -1 and U937, butonly little was detected in 6 colorectal cancer cell lines. CONCLUSION: The present data suggest that dThdPaseexpression is seldom detected in colorectal carcinoma cells. TAM is the most important source of dThdPase in colorectalcancer tissues.