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迟发型超敏反应(DTH)是代表T淋巴细胞免疫反应.以往报道测定小鼠DTH的方法是用特异抗原诱导,测定脚掌或耳肿胀来表示.(1,2,3,4,5)诱导DTH所用抗原包括:微生物及其产物,异种蛋白,多糖和简单化学接触剂.(3,6)最近Hartley(7)提出新的方法测定小鼠DTH.该方法用羊红细胞抗原致敏,然后在尾中部皮下进行攻击注射,DTH通过测定尾部肿胀程度来表示,此方法比测定脚掌,耳软组织的肿胀有高的重复性及可靠性.此外,近年认识到蛋白抗原用完全佐剂
Delayed-type hypersensitivity (DTH) represents the T-lymphocyte immune response. Previously reported methods for measuring DTH in mice were induced with specific antigens and measured for paw or ear swelling. (1,2,3,4,5) induction The antigens used by DTH include: microorganisms and their products, heterologous proteins, polysaccharides and simple chemical contact agents. (3,6) Recently Hartley (7) proposed a new method for the determination of mouse DTH. This method is sensitized with sheep red cell antigen and then In the mid-tail of the tail, the DTH is expressed by measuring the degree of swelling of the tail. This method is more reproducible and reliable than measuring the swelling of the soles of the feet and the soft tissue of the ear. In addition, complete antigens have been recognized as protein antigens in recent years.